Real lab failures, root causes, and fixes — curated and bilingually annotated by our team.
Presence of Bioanalyzer peaks smaller than 85 bp observed after PCR cleanup. These peaks represent residual primers from the amplification reaction.
Bioanalyzer shows an additional peak at higher molecular weight than expected library size (approximately 1,000 bp). This represents single-stranded library products that have self-annealed to form heteroduplexes.
Bioanalyzer shows a distinct peak at approximately 127 bp representing adaptor-dimer formation. These dimers will cluster and be sequenced, potentially consuming sequencing capacity.
Library shows a broad size distribution on Bioanalyzer with longer insert sizes than expected. This indicates heterogeneous fragment lengths in the final library.
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