Cell Passaging Steps and Precautions

critical

Mycoplasma Contamination - Silent Growth Inhibitor

Slowed cell growth, altered cellular metabolism, chromosomal aberrations, and interference with cell attachment. No visible turbidity in medium. Difficult to detect by light microscopy due to small size (0.15-0.3 µm).

💡 6 · ✓ 7

severe

Bacterial Contamination with Rapid Turbidity

Rapid-onset turbidity and color change of culture medium (when phenol red is present). Medium becomes cloudy and pH indicator changes color quickly.

💡 7 · ✓ 9

severe

Contamination from Aerosol Generation

Multiple cultures becoming contaminated simultaneously or in sequence. Contamination spreading despite apparently good technique. Cross-contamination between neighboring cultures.

💡 7 · ✓ 9