Qiagen RNA Extraction: Clean Results Protocol

severe

Low RNA Yield After Cleanup

Recovered RNA concentration is significantly lower than expected based on input amount. Spectrophotometric measurement shows inadequate RNA quantity for downstream applications.

💡 5 · ✓ 5

moderate

Unexpected or Incorrect Quantification Value

Qubit Fluorometer shows unexpected concentration values that do not match expected sample concentration

💡 10 · ✓ 10

moderate

Low A260/230 Ratio Indicates Contaminant Carryover

Spectrophotometric analysis shows A260/230 ratio below 1.8-2.0, indicating residual guanidine salt or other chaotropic agent contamination in the purified RNA.

💡 4 · ✓ 5