Gradient preparation
Stereotaxic surgery.
Injection of flies.
Treatment with collagenase and centrifugation
Example of a BALB/c mice performing reversal
Short tutorial for membrane-based 3D image segmentation using Imaris Cell.
Separate the common carotid artery from the Vagus and aortic depressor nerve
Larval body wall dissection at 3-fold time-lapse.
Intradermal inoculation of the mouse ear
Isolation of striatum from embryonic E16 mouse brain
Repository version control in R studio
Mouse dissection
Recording of the locomotor behavior of the flies
Procedure to install the Sice Spotdetector Macro and related Plugins
Mincing of adipose tissue
Separation of spores from sporangia
Example video of the experimental setup and analysis.
Image processing of the reference image to correct for uneven illumination
Calibration of the force applied to a 4.5 μm bead.
Procedure to find the best parameters to segment intracellular particles with the SiCE spot detector macro
Separation and sorting of the ovarioles
Image processing
Merging of all the acquired files together to create a linearized image of HCs using main1.m
Visualization of the protocol as a video recording of the procedures
Assembly and use of the setup
Zebrafish euthanasia and harvesting of the eye
Dissection procedure
Precision technique for mouse splenectomy.
Showing experimental protocol for the establishment of empathy for pain model in laboratory animals.
Flux vs. time
Steps to use the Kinetic Evaluation Software
Imaging of inner HCs using the main2.m program
Observational Parameters–Central Nervous System
Retinal injury in zebrafish
Movie showing how to measure mesocotyl lengths using FIJI
Fluorescence intensity measurement
Measurement of Fv/Fm
Olfactory Habituation Training and Testing
Gel electroelution protocol.
Procedure for the fruit injection as stated in Step 7
Electroporation procedure
Main steps of sample and slide preparation
Sample video of an entire play behavior test
Imaging of outer HCs using the main3.m program
Assembly of the transfer pack in a semi-dry blotter
Example video of a 60-s delay trial during the third stage of behavioral testing
Luciferin injection and image acquisition demonstration.
Accelerated snowmelt time-lapse during 2016-2017 snow season
Analysis of clustered HC loss using the main6.m program
Scraping
Quantification of binding using ImageJ and Excel
Harvest peritoneal macrophages
Video-EEG of a mouse exhibiting SE
Main steps of microscope slide preparation
Video of the partial optic nerve transection procedure.
Video of slide preparation
Video of dissection procedure
Tracking of microglial processes.
Demonstration of the T-maze construction
Movie showing how to peel and stain maize leaf epidermis following the pectolyase digestion step