Gradient preparation
Repository version control in R studio
Separation of spores from sporangia
Larval body wall dissection at 3-fold time-lapse.
Recording of the locomotor behavior of the flies
Calibration of the force applied to a 4.5 μm bead.
Procedure to find the best parameters to segment intracellular particles with the SiCE spot detector macro
Treatment with collagenase and centrifugation
Separate the common carotid artery from the Vagus and aortic depressor nerve
Intradermal inoculation of the mouse ear
Example video of the experimental setup and analysis.
Stereotaxic surgery.
Short tutorial for membrane-based 3D image segmentation using Imaris Cell.
Image processing of the reference image to correct for uneven illumination
Mouse dissection
Separation and sorting of the ovarioles
Injection of flies.
Isolation of striatum from embryonic E16 mouse brain
Example of a BALB/c mice performing reversal
Mincing of adipose tissue
Procedure to install the Sice Spotdetector Macro and related Plugins
Main steps of microscope slide preparation
Movie showing how to measure mesocotyl lengths using FIJI
Merging of all the acquired files together to create a linearized image of HCs using main1.m
Video-EEG of a mouse exhibiting SE
Assembly and use of the setup
Quantification of binding using ImageJ and Excel
Imaging of inner HCs using the main2.m program
Showing experimental protocol for the establishment of empathy for pain model in laboratory animals.
Observational Parameters–Central Nervous System
Procedure for the fruit injection as stated in Step 7
Main steps of sample and slide preparation
Harvest peritoneal macrophages
Visualization of the protocol as a video recording of the procedures
Flux vs. time
Olfactory Habituation Training and Testing
Tracking of microglial processes.
Steps to use the Kinetic Evaluation Software
Fluorescence intensity measurement
Luciferin injection and image acquisition demonstration.
Electroporation procedure
Assembly of the transfer pack in a semi-dry blotter
Demonstration of the T-maze construction
Analysis of clustered HC loss using the main6.m program
Scraping
Example video of a 60-s delay trial during the third stage of behavioral testing
Dissection procedure
Imaging of outer HCs using the main3.m program
Video of dissection procedure
Sample video of an entire play behavior test
Gel electroelution protocol.
Video of slide preparation
Retinal injury in zebrafish
Measurement of Fv/Fm
Video of the partial optic nerve transection procedure.
Zebrafish euthanasia and harvesting of the eye
Precision technique for mouse splenectomy.
Accelerated snowmelt time-lapse during 2016-2017 snow season
Image processing
Disassembly and gel removal