Gradient preparation
Procedure to find the best parameters to segment intracellular particles with the SiCE spot detector macro
Separate the common carotid artery from the Vagus and aortic depressor nerve
Mincing of adipose tissue
Treatment with collagenase and centrifugation
Stereotaxic surgery.
Procedure to install the Sice Spotdetector Macro and related Plugins
Image processing of the reference image to correct for uneven illumination
Recording of the locomotor behavior of the flies
Injection of flies.
Larval body wall dissection at 3-fold time-lapse.
Separation and sorting of the ovarioles
Example of a BALB/c mice performing reversal
Intradermal inoculation of the mouse ear
Calibration of the force applied to a 4.5 μm bead.
Short tutorial for membrane-based 3D image segmentation using Imaris Cell.
Separation of spores from sporangia
Mouse dissection
Repository version control in R studio
Example video of the experimental setup and analysis.
Isolation of striatum from embryonic E16 mouse brain
Tracking of microglial processes.
Example video of a 60-s delay trial during the third stage of behavioral testing
Luciferin injection and image acquisition demonstration.
Dissection procedure
Retinal injury in zebrafish
Precision technique for mouse splenectomy.
Accelerated snowmelt time-lapse during 2016-2017 snow season
Sample video of an entire play behavior test
Main steps of microscope slide preparation
Fluorescence intensity measurement
Visualization of the protocol as a video recording of the procedures
Video of the partial optic nerve transection procedure.
Video of dissection procedure
Flux vs. time
Measurement of Fv/Fm
Imaging of outer HCs using the main3.m program
Imaging of inner HCs using the main2.m program
Gel electroelution protocol.
Olfactory Habituation Training and Testing
Video of slide preparation
Image processing
Observational Parameters–Central Nervous System
Movie showing how to measure mesocotyl lengths using FIJI
Main steps of sample and slide preparation
Electroporation procedure
Zebrafish euthanasia and harvesting of the eye
Scraping
Assembly of the transfer pack in a semi-dry blotter
Demonstration of the T-maze construction
Showing experimental protocol for the establishment of empathy for pain model in laboratory animals.
Steps to use the Kinetic Evaluation Software
Merging of all the acquired files together to create a linearized image of HCs using main1.m
Analysis of clustered HC loss using the main6.m program
Assembly and use of the setup
Harvest peritoneal macrophages
Video-EEG of a mouse exhibiting SE
Procedure for the fruit injection as stated in Step 7
Quantification of binding using ImageJ and Excel
Trace Fear Conditioning Protocol.